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tom70 mcherry frb  (Addgene inc)


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    Addgene inc tom70 mcherry frb
    Additional file 5: Video 4 RNb-FKBP rapidly sequesters an ER integral membrane protein at the OMM. TIRFM images of HeLa cells expressing TOM70-GFP-FRB, RNb-FKBP and mCh-Sec61β were treated with rapamycin (100 nM, added at 60 s). The ER membrane protein, mCh-Sec61β, is then rapidly sequestered at the OMM. The video was acquired at 0.5 Hz and shown at 33 fps. The clock is in min:s. Relates to Fig. <xref ref-type=10 c, d. (MP4 551 kb) " width="250" height="auto" />
    Tom70 Mcherry Frb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tom70+mcherry+frb/pmTurquoise2-Golgi+(Plasmid+%2336205)/pmc06533734-312-98-100
    Average 94 stars, based on 46 article reviews
    tom70 mcherry frb - by Bioz Stars, 2026-09
    94/100 stars

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    1) Product Images from "A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling"

    Article Title: A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling

    Journal: BMC Biology

    doi: 10.1186/s12915-019-0662-4

    Additional file 5: Video 4 RNb-FKBP rapidly sequesters an ER integral membrane protein at the OMM. TIRFM images of HeLa cells expressing TOM70-GFP-FRB, RNb-FKBP and mCh-Sec61β were treated with rapamycin (100 nM, added at 60 s). The ER membrane protein, mCh-Sec61β, is then rapidly sequestered at the OMM. The video was acquired at 0.5 Hz and shown at 33 fps. The clock is in min:s. Relates to Fig. <xref ref-type=10 c, d. (MP4 551 kb) " title="Additional file 5: Video 4 RNb-FKBP rapidly sequesters an ER integral membrane protein" property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Additional file 5: Video 4 RNb-FKBP rapidly sequesters an ER integral membrane protein at the OMM. TIRFM images of HeLa cells expressing TOM70-GFP-FRB, RNb-FKBP and mCh-Sec61β were treated with rapamycin (100 nM, added at 60 s). The ER membrane protein, mCh-Sec61β, is then rapidly sequestered at the OMM. The video was acquired at 0.5 Hz and shown at 33 fps. The clock is in min:s. Relates to Fig. 10 c, d. (MP4 551 kb)

    Techniques Used:

    Additional file 6: Video 5. GNb-FKBP rapidly sequesters endogenously tagged GFP-IP 3 R1 at the OMM. TIRFM images show HeLa cells with endogenously GFP-tagged IP 3 R1 and transiently expressing TOM70-mCh-FRB and GNb-FKBP, and then treated with rapamycin (100 nM, added at 60 s). GFP-IP 3 R1 is rapidly sequestered at the OMM. The video was acquired at 0.5 Hz and is shown at 33 fps. The clock is in min:s. Relates to Fig. <xref ref-type=10 f and g. (MP4 2601 kb) " title="Additional file 6: Video 5. GNb-FKBP rapidly sequesters endogenously tagged GFP-IP 3 R1" property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Additional file 6: Video 5. GNb-FKBP rapidly sequesters endogenously tagged GFP-IP 3 R1 at the OMM. TIRFM images show HeLa cells with endogenously GFP-tagged IP 3 R1 and transiently expressing TOM70-mCh-FRB and GNb-FKBP, and then treated with rapamycin (100 nM, added at 60 s). GFP-IP 3 R1 is rapidly sequestered at the OMM. The video was acquired at 0.5 Hz and is shown at 33 fps. The clock is in min:s. Relates to Fig. 10 f and g. (MP4 2601 kb)

    Techniques Used:

    Additional file 8: Video 7. RNb-FKBP recruits a PM protein to the OMM in response to rapamycin. TIRFM images of HeLa cells expressing TOM70-GFP-FRB, RNb-FKBP and the PM protein, β 2 AR-mCh, and then exposed to rapamycin (100 nM, added at 60 s). There is a rapid translocation of β 2 AR-mCh to the OMM. The video was acquired at 0.5 Hz and is shown at 33 fps. The clock is in min:s. Relates to Fig. <xref ref-type=12 b–e. (MP4 2141 kb) " title="Additional file 8: Video 7. RNb-FKBP recruits a PM protein to the OMM" property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Additional file 8: Video 7. RNb-FKBP recruits a PM protein to the OMM in response to rapamycin. TIRFM images of HeLa cells expressing TOM70-GFP-FRB, RNb-FKBP and the PM protein, β 2 AR-mCh, and then exposed to rapamycin (100 nM, added at 60 s). There is a rapid translocation of β 2 AR-mCh to the OMM. The video was acquired at 0.5 Hz and is shown at 33 fps. The clock is in min:s. Relates to Fig. 12 b–e. (MP4 2141 kb)

    Techniques Used:

    Related Articles

    Membrane:

    Article Title: A genetically-encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling
    Article Snippet: Other reagents, including histamine and nigericin, were from Sigma-Aldrich. .. Sources of plasmids encoding the following proteins were: mCherry-C1 (Clontech #632524); mCherry-N1 (Clontech #632523); EGFP-N1 (Clontech #6085-1); GFP-ERcyt, mCherry-ERcyt and mTurquoise2-ERcyt (GFP, mCherry or mTurquoise2 targeted to the cytosolic side of the ER membrane via the ER-targeting sequence of the yeast UBC6 protein) [ ]; mCherry-ERlumen (Addgene #55041, provided by Michael Davidson); LAMP1-mCherry [ ]; TPC2-mRFP [ ]; TOM20-mCherry (Addgene #55146, provided by Michael Davidson); CIB1-mRFP-MP (Addgene #58367) [ ]; CIB1-mCerulean-MP (Addgene #58366) [ ]; H2B-GFP (Addgene #11680) [ ]; TOM20-LOV2 (Addgene #81009) [ ]; mCherry-Sec61β [ ]; GFP-MAPPER [ ]; GFP-CaM (Addgene #47602, provided by Emanuel Strehler); TOM70-mCherry-FRB (pMito-mCherry-FRB, Addgene #59352) [ ]; pmTurquoise2-Golgi (Addgene #36205) [ ]; pTriEx-mCherry-zdk1 (Addgene #81057) [ ]; pTriEx-NTOM20-LOV2 (Addgene #81009) [ ]; β 2 AR-mCFP (Addgene #38260) [ ]; pCMV-G-CEPIA1er (Addgene #58215) [ ]; pCMV-R-CEPIA1er (Addgene #58216) [ ]; pCIS-GEM-CEPIA1er (Addgene #58217) [ ]; CMV-ER-LAR-GECO1 and CMV-mito-LAR-GECO1.2 [ ]; mCherry-MAPPER and mCherry-Orai1 [ ]. .. H2B-mCh was made by transferring H2B from H2B-GFP to pmCherry-N1 (Clontech) using Kpn I/ Bam HI.

    Article Title: A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling
    Article Snippet: Other reagents, including histamine and nigericin, were from Sigma-Aldrich. .. Sources of plasmids encoding the following proteins were mCherry-C1 (Clontech #632524); mCherry-N1 (Clontech #632523); EGFP-N1 (Clontech #6085-1); GFP-ERcyt, mCherry-ERcyt and mTurquoise2-ERcyt (GFP, mCherry or mTurquoise2 targeted to the cytosolic side of the ER membrane via the ER-targeting sequence of the yeast UBC6 protein) [ ]; mCherry-ERlumen (Addgene #55041, provided by Michael Davidson); LAMP1-mCherry [ ]; TPC2-mRFP [ ]; TOM20-mCherry (Addgene #55146, provided by Michael Davidson); CIB1-mRFP-MP (Addgene #58367) [ ]; CIB1-mCerulean-MP (Addgene #58366) [ ]; H2B-GFP (Addgene #11680) [ ]; TOM20-LOV2 (Addgene #81009) [ ]; mCherry-Sec61β [ ]; GFP-MAPPER [ ]; GFP-CaM (Addgene #47602, provided by Emanuel Strehler); TOM70-mCherry-FRB (pMito-mCherry-FRB, Addgene #59352) [ ]; pmTurquoise2-Golgi (Addgene #36205) [ ]; pTriEx-mCherry-zdk1 (Addgene #81057) [ ]; pTriEx-NTOM20-LOV2 (Addgene #81009) [ ]; β 2 AR-mCFP (Addgene #38260) [ ]; pCMV-G-CEPIA1er (Addgene #58215) [ ]; pCMV-R-CEPIA1er (Addgene #58216) [ ]; pCIS-GEMCEPIA1er (Addgene #58217) [ ]; CMV-ER-LAR-GECO1 and CMV-mito-LAR-GECO1.2 [ ]; mCherry-MAPPER and mCherry-Orai1 [ ]. .. H2B-mCh was made by transferring H2B from H2B-GFP to pmCherry-N1 (Clontech) using Kpn I/ Bam HI.

    Sequencing:

    Article Title: A genetically-encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling
    Article Snippet: Other reagents, including histamine and nigericin, were from Sigma-Aldrich. .. Sources of plasmids encoding the following proteins were: mCherry-C1 (Clontech #632524); mCherry-N1 (Clontech #632523); EGFP-N1 (Clontech #6085-1); GFP-ERcyt, mCherry-ERcyt and mTurquoise2-ERcyt (GFP, mCherry or mTurquoise2 targeted to the cytosolic side of the ER membrane via the ER-targeting sequence of the yeast UBC6 protein) [ ]; mCherry-ERlumen (Addgene #55041, provided by Michael Davidson); LAMP1-mCherry [ ]; TPC2-mRFP [ ]; TOM20-mCherry (Addgene #55146, provided by Michael Davidson); CIB1-mRFP-MP (Addgene #58367) [ ]; CIB1-mCerulean-MP (Addgene #58366) [ ]; H2B-GFP (Addgene #11680) [ ]; TOM20-LOV2 (Addgene #81009) [ ]; mCherry-Sec61β [ ]; GFP-MAPPER [ ]; GFP-CaM (Addgene #47602, provided by Emanuel Strehler); TOM70-mCherry-FRB (pMito-mCherry-FRB, Addgene #59352) [ ]; pmTurquoise2-Golgi (Addgene #36205) [ ]; pTriEx-mCherry-zdk1 (Addgene #81057) [ ]; pTriEx-NTOM20-LOV2 (Addgene #81009) [ ]; β 2 AR-mCFP (Addgene #38260) [ ]; pCMV-G-CEPIA1er (Addgene #58215) [ ]; pCMV-R-CEPIA1er (Addgene #58216) [ ]; pCIS-GEM-CEPIA1er (Addgene #58217) [ ]; CMV-ER-LAR-GECO1 and CMV-mito-LAR-GECO1.2 [ ]; mCherry-MAPPER and mCherry-Orai1 [ ]. .. H2B-mCh was made by transferring H2B from H2B-GFP to pmCherry-N1 (Clontech) using Kpn I/ Bam HI.

    Article Title: A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling
    Article Snippet: Other reagents, including histamine and nigericin, were from Sigma-Aldrich. .. Sources of plasmids encoding the following proteins were mCherry-C1 (Clontech #632524); mCherry-N1 (Clontech #632523); EGFP-N1 (Clontech #6085-1); GFP-ERcyt, mCherry-ERcyt and mTurquoise2-ERcyt (GFP, mCherry or mTurquoise2 targeted to the cytosolic side of the ER membrane via the ER-targeting sequence of the yeast UBC6 protein) [ ]; mCherry-ERlumen (Addgene #55041, provided by Michael Davidson); LAMP1-mCherry [ ]; TPC2-mRFP [ ]; TOM20-mCherry (Addgene #55146, provided by Michael Davidson); CIB1-mRFP-MP (Addgene #58367) [ ]; CIB1-mCerulean-MP (Addgene #58366) [ ]; H2B-GFP (Addgene #11680) [ ]; TOM20-LOV2 (Addgene #81009) [ ]; mCherry-Sec61β [ ]; GFP-MAPPER [ ]; GFP-CaM (Addgene #47602, provided by Emanuel Strehler); TOM70-mCherry-FRB (pMito-mCherry-FRB, Addgene #59352) [ ]; pmTurquoise2-Golgi (Addgene #36205) [ ]; pTriEx-mCherry-zdk1 (Addgene #81057) [ ]; pTriEx-NTOM20-LOV2 (Addgene #81009) [ ]; β 2 AR-mCFP (Addgene #38260) [ ]; pCMV-G-CEPIA1er (Addgene #58215) [ ]; pCMV-R-CEPIA1er (Addgene #58216) [ ]; pCIS-GEMCEPIA1er (Addgene #58217) [ ]; CMV-ER-LAR-GECO1 and CMV-mito-LAR-GECO1.2 [ ]; mCherry-MAPPER and mCherry-Orai1 [ ]. .. H2B-mCh was made by transferring H2B from H2B-GFP to pmCherry-N1 (Clontech) using Kpn I/ Bam HI.



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    tom70 mcherry frb  (Addgene inc)
    94
    Addgene inc tom70 mcherry frb
    Additional file 5: Video 4 RNb-FKBP rapidly sequesters an ER integral membrane protein at the OMM. TIRFM images of HeLa cells expressing TOM70-GFP-FRB, RNb-FKBP and mCh-Sec61β were treated with rapamycin (100 nM, added at 60 s). The ER membrane protein, mCh-Sec61β, is then rapidly sequestered at the OMM. The video was acquired at 0.5 Hz and shown at 33 fps. The clock is in min:s. Relates to Fig. <xref ref-type=10 c, d. (MP4 551 kb) " width="250" height="auto" />
    Tom70 Mcherry Frb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tom70+mcherry+frb/pmTurquoise2-Golgi+(Plasmid+%2336205)/pmc06533734-312-98-100
    Average 94 stars, based on 1 article reviews
    tom70 mcherry frb - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Additional file 5: Video 4 RNb-FKBP rapidly sequesters an ER integral membrane protein at the OMM. TIRFM images of HeLa cells expressing TOM70-GFP-FRB, RNb-FKBP and mCh-Sec61β were treated with rapamycin (100 nM, added at 60 s). The ER membrane protein, mCh-Sec61β, is then rapidly sequestered at the OMM. The video was acquired at 0.5 Hz and shown at 33 fps. The clock is in min:s. Relates to Fig. <xref ref-type=10 c, d. (MP4 551 kb) " width="100%" height="100%">

    Journal: BMC Biology

    Article Title: A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling

    doi: 10.1186/s12915-019-0662-4

    Figure Lengend Snippet: Additional file 5: Video 4 RNb-FKBP rapidly sequesters an ER integral membrane protein at the OMM. TIRFM images of HeLa cells expressing TOM70-GFP-FRB, RNb-FKBP and mCh-Sec61β were treated with rapamycin (100 nM, added at 60 s). The ER membrane protein, mCh-Sec61β, is then rapidly sequestered at the OMM. The video was acquired at 0.5 Hz and shown at 33 fps. The clock is in min:s. Relates to Fig. 10 c, d. (MP4 551 kb)

    Article Snippet: Sources of plasmids encoding the following proteins were mCherry-C1 (Clontech #632524); mCherry-N1 (Clontech #632523); EGFP-N1 (Clontech #6085-1); GFP-ERcyt, mCherry-ERcyt and mTurquoise2-ERcyt (GFP, mCherry or mTurquoise2 targeted to the cytosolic side of the ER membrane via the ER-targeting sequence of the yeast UBC6 protein) [ ]; mCherry-ERlumen (Addgene #55041, provided by Michael Davidson); LAMP1-mCherry [ ]; TPC2-mRFP [ ]; TOM20-mCherry (Addgene #55146, provided by Michael Davidson); CIB1-mRFP-MP (Addgene #58367) [ ]; CIB1-mCerulean-MP (Addgene #58366) [ ]; H2B-GFP (Addgene #11680) [ ]; TOM20-LOV2 (Addgene #81009) [ ]; mCherry-Sec61β [ ]; GFP-MAPPER [ ]; GFP-CaM (Addgene #47602, provided by Emanuel Strehler); TOM70-mCherry-FRB (pMito-mCherry-FRB, Addgene #59352) [ ]; pmTurquoise2-Golgi (Addgene #36205) [ ]; pTriEx-mCherry-zdk1 (Addgene #81057) [ ]; pTriEx-NTOM20-LOV2 (Addgene #81009) [ ]; β 2 AR-mCFP (Addgene #38260) [ ]; pCMV-G-CEPIA1er (Addgene #58215) [ ]; pCMV-R-CEPIA1er (Addgene #58216) [ ]; pCIS-GEMCEPIA1er (Addgene #58217) [ ]; CMV-ER-LAR-GECO1 and CMV-mito-LAR-GECO1.2 [ ]; mCherry-MAPPER and mCherry-Orai1 [ ].

    Techniques:

    Additional file 6: Video 5. GNb-FKBP rapidly sequesters endogenously tagged GFP-IP 3 R1 at the OMM. TIRFM images show HeLa cells with endogenously GFP-tagged IP 3 R1 and transiently expressing TOM70-mCh-FRB and GNb-FKBP, and then treated with rapamycin (100 nM, added at 60 s). GFP-IP 3 R1 is rapidly sequestered at the OMM. The video was acquired at 0.5 Hz and is shown at 33 fps. The clock is in min:s. Relates to Fig. <xref ref-type=10 f and g. (MP4 2601 kb) " width="100%" height="100%">

    Journal: BMC Biology

    Article Title: A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling

    doi: 10.1186/s12915-019-0662-4

    Figure Lengend Snippet: Additional file 6: Video 5. GNb-FKBP rapidly sequesters endogenously tagged GFP-IP 3 R1 at the OMM. TIRFM images show HeLa cells with endogenously GFP-tagged IP 3 R1 and transiently expressing TOM70-mCh-FRB and GNb-FKBP, and then treated with rapamycin (100 nM, added at 60 s). GFP-IP 3 R1 is rapidly sequestered at the OMM. The video was acquired at 0.5 Hz and is shown at 33 fps. The clock is in min:s. Relates to Fig. 10 f and g. (MP4 2601 kb)

    Article Snippet: Sources of plasmids encoding the following proteins were mCherry-C1 (Clontech #632524); mCherry-N1 (Clontech #632523); EGFP-N1 (Clontech #6085-1); GFP-ERcyt, mCherry-ERcyt and mTurquoise2-ERcyt (GFP, mCherry or mTurquoise2 targeted to the cytosolic side of the ER membrane via the ER-targeting sequence of the yeast UBC6 protein) [ ]; mCherry-ERlumen (Addgene #55041, provided by Michael Davidson); LAMP1-mCherry [ ]; TPC2-mRFP [ ]; TOM20-mCherry (Addgene #55146, provided by Michael Davidson); CIB1-mRFP-MP (Addgene #58367) [ ]; CIB1-mCerulean-MP (Addgene #58366) [ ]; H2B-GFP (Addgene #11680) [ ]; TOM20-LOV2 (Addgene #81009) [ ]; mCherry-Sec61β [ ]; GFP-MAPPER [ ]; GFP-CaM (Addgene #47602, provided by Emanuel Strehler); TOM70-mCherry-FRB (pMito-mCherry-FRB, Addgene #59352) [ ]; pmTurquoise2-Golgi (Addgene #36205) [ ]; pTriEx-mCherry-zdk1 (Addgene #81057) [ ]; pTriEx-NTOM20-LOV2 (Addgene #81009) [ ]; β 2 AR-mCFP (Addgene #38260) [ ]; pCMV-G-CEPIA1er (Addgene #58215) [ ]; pCMV-R-CEPIA1er (Addgene #58216) [ ]; pCIS-GEMCEPIA1er (Addgene #58217) [ ]; CMV-ER-LAR-GECO1 and CMV-mito-LAR-GECO1.2 [ ]; mCherry-MAPPER and mCherry-Orai1 [ ].

    Techniques:

    Additional file 8: Video 7. RNb-FKBP recruits a PM protein to the OMM in response to rapamycin. TIRFM images of HeLa cells expressing TOM70-GFP-FRB, RNb-FKBP and the PM protein, β 2 AR-mCh, and then exposed to rapamycin (100 nM, added at 60 s). There is a rapid translocation of β 2 AR-mCh to the OMM. The video was acquired at 0.5 Hz and is shown at 33 fps. The clock is in min:s. Relates to Fig. <xref ref-type=12 b–e. (MP4 2141 kb) " width="100%" height="100%">

    Journal: BMC Biology

    Article Title: A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling

    doi: 10.1186/s12915-019-0662-4

    Figure Lengend Snippet: Additional file 8: Video 7. RNb-FKBP recruits a PM protein to the OMM in response to rapamycin. TIRFM images of HeLa cells expressing TOM70-GFP-FRB, RNb-FKBP and the PM protein, β 2 AR-mCh, and then exposed to rapamycin (100 nM, added at 60 s). There is a rapid translocation of β 2 AR-mCh to the OMM. The video was acquired at 0.5 Hz and is shown at 33 fps. The clock is in min:s. Relates to Fig. 12 b–e. (MP4 2141 kb)

    Article Snippet: Sources of plasmids encoding the following proteins were mCherry-C1 (Clontech #632524); mCherry-N1 (Clontech #632523); EGFP-N1 (Clontech #6085-1); GFP-ERcyt, mCherry-ERcyt and mTurquoise2-ERcyt (GFP, mCherry or mTurquoise2 targeted to the cytosolic side of the ER membrane via the ER-targeting sequence of the yeast UBC6 protein) [ ]; mCherry-ERlumen (Addgene #55041, provided by Michael Davidson); LAMP1-mCherry [ ]; TPC2-mRFP [ ]; TOM20-mCherry (Addgene #55146, provided by Michael Davidson); CIB1-mRFP-MP (Addgene #58367) [ ]; CIB1-mCerulean-MP (Addgene #58366) [ ]; H2B-GFP (Addgene #11680) [ ]; TOM20-LOV2 (Addgene #81009) [ ]; mCherry-Sec61β [ ]; GFP-MAPPER [ ]; GFP-CaM (Addgene #47602, provided by Emanuel Strehler); TOM70-mCherry-FRB (pMito-mCherry-FRB, Addgene #59352) [ ]; pmTurquoise2-Golgi (Addgene #36205) [ ]; pTriEx-mCherry-zdk1 (Addgene #81057) [ ]; pTriEx-NTOM20-LOV2 (Addgene #81009) [ ]; β 2 AR-mCFP (Addgene #38260) [ ]; pCMV-G-CEPIA1er (Addgene #58215) [ ]; pCMV-R-CEPIA1er (Addgene #58216) [ ]; pCIS-GEMCEPIA1er (Addgene #58217) [ ]; CMV-ER-LAR-GECO1 and CMV-mito-LAR-GECO1.2 [ ]; mCherry-MAPPER and mCherry-Orai1 [ ].

    Techniques: